Course Overview:

Duration : 2 Month

• Laboratory safety manuals
• Hands own experience in nucleic acid extraction
• Hands on experience in Real time PCR

 You will Gain:

• Gain in-depth understanding of molecular laboratory practices and safety guidelines.
• Gain an in-depth understanding of advanced molecular biology techniques and their applications.
• Hands-on experience with nucleic acid extraction
• Hands own experience in quality check of extracted nucleic acid with different methods.
• Acquire skills in Real Time Polymerase Chain Reaction and Reverse Transcriptase PCR.
• Data analysis.
• Interact with experienced instructors and fellow participants.
• Receive a Certificate of Completion.

 You will go through:

 Introduction to Molecular Biology Lab

• Lab Structure
• Safety routines in Molecular diagnostic laboratories.
• Introduction to Lab Equipment and their maintenance
• Basics of Molecular Tests

2. Nucleic Acid Extraction

• DNA extraction from Blood
• DNA extraction from FFPE tissue
• RNA extraction from Blood

3. Quantification and Quality Check of Nucleic Acids

• DNA and RNA Quantification by Nano Drop Spectrophotometer
• DNA and RNA Quantification by Qubit Fluorometer
• Nucleic Acid Quality Analysis using Gel Electrophoresis

4. PCR Amplification

• cDNA Synthesis from Extracted RNA
• PCR Amplification of DNA
• Gel Electrophoresis of amplified products

5. Real Time PCR Technique

• Real Time PCR Amplification
• Data Analysis

Who Should Attend:

• Researchers, professionals, and students with a background in molecular biology.
• Researchers and practitioners seeking to expand their knowledge of advanced techniques.
• Individuals interested in gene expression analysis and bioinformatics for genomic data analysis.