Course Overview

Duration : 1 month

• Laboratory safety manuals
• Hands own experience in nucleic acid extraction
• Hands own experience in nucleic acid amplification and detection.

You will Gain:

• Gain in-depth understanding of molecular laboratory practices and safety guidelines.
• Gain an in-depth understanding of advanced molecular biology techniques and their applications.
• Hands-on experience with nucleic acid extraction
• Hands own experience in quality check of extracted nucleic acid.
• Acquire skills in polymerase chain reaction.
• Interact with experienced instructors and fellow participants.
• Receive a Certificate of Completion.

You will go through:

1. Introduction to Molecular Biology Lab
   • Lab Structure
   • Safety routines in Molecular diagnostic laboratories.
   • Introduction to Lab equipment and their maintenance
   • Basics of Molecular Tests
2. Nucleic Acid Extraction
   • DNA extraction from Blood
   • DNA extraction from FFPE tissue
   • RNA extraction from Blood
3. Quantification and Quality Check of Nucleic Acids
   • DNA and RNA Quantification by Nano Drop Spectrophotometer
4. PCR Amplification
   • cDNA Synthesis from Extracted RNA
   • PCR Amplification of DNA
   • Gel Electrophoresis of amplified products

Who Should Attend:

• Researchers, professionals, and students with a background in molecular biology.
• Researchers and practitioners seeking to expand their knowledge of advanced techniques.
• Individuals interested in gene expression analysis and bioinformatics for genomic data analysis.